型号：GM-J#037459 -Available For Pre-Order

应用：Lmx1a-Cre transgenic mice express a Cre recombinase/EGFP fusion under the control of the mouse Lmx1a (LIM homeobox transcription factor 1 alpha) promoter elements. Please note: the transgene inserted on the X chromosome. Cre recombinase activity is observed specifically in the roof plate (RF) and a subset of rhombic lip (RL) progenitors. This strain may be useful in fate mapping and manipulating gene expression in multiple Lmx1a populations.

型号：GM-J#037456 -Available

应用：mT/mG (also called ROSAmT/mG) is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence.

型号：GM-J#037455 -Cryorecovery

应用：The granzyme-B-Cre transgene is designed to have the human GZMB (granzyme B) promoter directing Cre recombinase expression to activated T cells.

型号：GM-J#037395 -Available

应用：R26 BirA*G3 knock-in mice have a CAG promoter controlling the Cre-inducible expression of a myc-tagged endoplasmic reticulum-directed promiscuous biotin ligase sequence, BirA*G3-ER. Cre-mediated recombination results in the excision of a floxed GFP sequence, enabling the tissue and time-dependent expression of BirA*G3 and mKate2. This is a tool for the isolation and purification of proteins and protein complexes trafficking through the secretory pathway upon biotin administration.

型号：GM-J#037382 -Available

应用：Ai224 (also called Ai224 (TICL-nls-EGFP-ICF-nls-dTom)) is a dual-recombinase responsive reporter allele designed with a CAG promoter, loxP-STOP-loxP and EGFP coding sequence with a nuclear localization sequence followed by a CAG promoter, frt-STOP-frt and dTomato coding sequence with a nuclear localization sequence - all inserted into the Igs7 locus (TIGRE). Exposure to either Cre or FLP recombinase results in nuclear localized EGFP fluorescence or nuclear localized dTomato fluorescence, respectively. Furthermore, Ai224 are a Cre::FLP intersectional fluorescent reporter line as they are designed to allow both nuclear localized EGFP and nuclear localized dTomato fluorescence in those cells where Cre and FLP expression overlaps.

型号：GM-J#037381 -Cryorecovery

应用：Ai215 (also called Ai215 (TICL-stGtACR2-HA-ICL-GCaMP7s-SS)) is a Cre-dependent optogenetic and fluorescent calcium-indicator tool allele designed with a CAG promoter, loxP-STOP-loxP and stGtACR2 coding sequence followed by a CAG promoter, lox2272-STOP-lox2272 and GCaMP7s coding sequence - all inserted into the Igs7 locus (TIGRE). Exposure to Cre recombinase results in expression of stGtACR2 (soma-targeted variant of the red-shifted, light-gated anion channelrhodopsin GtACR2; neuronal silencing) and/or GCaMP7s fluorescence.

型号：GM-J#037380 -Available

应用：Ai214 (also called Ai214 (TICL-stGtACR1-HA-ICL-mRuby3)) is a Cre-dependent optogenetic and fluorescent tool allele designed with a CAG promoter, loxP-STOP-loxP and stGtACR1 coding sequence followed by a CAG promoter, lox2272-STOP-lox2272 and mRuby3 coding sequence - all inserted into the Igs7 locus (TIGRE). Exposure to Cre recombinase results in expression of stGtACR1 (soma-targeted variant of the red-shifted, light-gated anion channelrhodopsin GtACR1; neuronal silencing) and/or mRuby3 fluorescence.

型号：GM-J#037379 -Cryorecovery

应用：Ai211 (also called Ai211(TITL-ChrimsonR-tdT-Kv2.1-ICF-IRES-tTA2)) is a dual-recombinase responsive (cre/flp-dependent), Tet-controllable, fluorescent and optogenetic tool allele created by targeted insertion at the Igs7 locus (TIGRE). Exposure to both Cre and Flp recombinase results in expression of tTA2 and ChrR-tdT (a light-gated cation channel; neuronal activation) in those cells where cre and flp expression overlaps/intersects. ChrR-tdT expression may be expected to be diminished/eliminated by doxycycline.

型号：GM-J#037378 -Cryorecovery

应用：Ai210 (also called Ai210 (TITL-GC7f-ICF-IRES-tTA2)) is a dual-recombinase responsive (cre/flp-dependent), Tet-controllable, fluorescent calcium-indicator tool allele created by targeted insertion at the Igs7 locus (TIGRE). Exposure to both Cre and FLP recombinase results in expression of tTA2 and jGCaMP7f (an ultrasensitive EGFP fluorescent calcium detector of neuronal activity with faster decay and response kinetics) in those cells where cre and FLP expression overlaps/intersects. The jGCaMP7f expression may be expected to be diminished/eliminated by doxycycline. jGCaMP7f has high sensitivity and affinity, improved detection of individual spikes and may allow tracking of larger populations of neurons using two-photon imaging.

型号：GM-J#037337 -Cryorecovery

应用：Slco2a1-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous solute carrier organic anion transporter family member 2a1 promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Slco2a1-expressing cells.