产品型号:GM-J#037378 -Cryorecovery
产品应用:Ai210 (also called Ai210 (TITL-GC7f-ICF-IRES-tTA2)) is a dual-recombinase responsive (cre/flp-dependent), Tet-controllable, fluorescent calcium-indicator tool allele created by targeted insertion at the Igs7 locus (TIGRE). Exposure to both Cre and FLP recombinase results in expression of tTA2 and jGCaMP7f (an ultrasensitive EGFP fluorescent calcium detector of neuronal activity with faster decay and response kinetics) in those cells where cre and FLP expression overlaps/intersects. The jGCaMP7f expression may be expected to be diminished/eliminated by doxycycline. jGCaMP7f has high sensitivity and affinity, improved detection of individual spikes and may allow tracking of larger populations of neurons using two-photon imaging.