型号：GM-J#034113 -Cryorecovery

应用：Ai213 (also called Ai213(TICL-EGFP-ICF-mOrange2-ICN-mKate2)) is a triple-recombinase responsive reporter allele designed with a CAG promoter, loxP-STOP-loxP and EGFP coding sequence, followed by a CAG promoter, frt-STOP-frt and mOrange2 coding sequence which is followed by a CAG promoter, nox-STOP-nox and mKate2 coding sequence - all inserted into the Igs7 locus (TIGRE). Exposure to either Cre, FLP or Nigri recombinase results in EGFP fluorescence, mOrange2 fluorescence or mKate2 fluorescence, respectively. In total, Ai213 mice are a Cre::FLP::Nigri intersectional fluorescent reporter line designed to allow EGFP/mOrange2/mKate2 fluorescence in those cells where Cre, FLP and Nigri expression overlaps.

型号：GM-J#034112 -Available

应用：Ai195 (also called Ai195(TIT2L-GCaMP7s-ICF-IRES-tTA2)) is a dual-recombinase responsive (Cre/FLP-dependent), Tet-controllable, fluorescent calcium-indicator tool allele created by targeted insertion at the Igs7 locus (TIGRE). Exposure to both Cre and FLP recombinase results in expression of tTA2 and jGCaMP7s (an ultrasensitive EGFP fluorescent calcium detector of neuronal activity with slower decay and response kinetics) in those cells where Cre and FLP expression overlaps/intersects. The jGCaMP7s expression may be expected to be diminished/eliminated by doxycycline. jGCaMP7s has high sensitivity and affinity, improved detection of individual spikes and may allow tracking larger populations of neurons using two-photon imaging.

型号：GM-J#034111 -Cryorecovery

应用：Ai193 (also called Ai193(TICL-EGFP-ICF-tdT)) is a dual-recombinase responsive reporter allele designed with a CAG promoter, loxP-STOP-loxP and EGFP coding sequence followed by a CAG promoter, frt-STOP-frt and tdTomato coding sequence - all inserted into the Igs7 locus (TIGRE). Exposure to either Cre or FLP recombinase results in EGFP fluorescence or tdTomato fluorescence, respectively. Furthermore, Ai193 are a Cre::FLP intersectional fluorescent reporter line as they are designed to allow both EGFP and tdTomato fluorescence in those cells where Cre and FLP expression overlaps.

型号：GM-J#034103 -Cryorecovery

应用：Ai175 (also called Ai175(TIT2L-EGFP-shOct4-ICL-tdT-tTA2)) is a Cre-dependent, Tet-controllable transcription tool strain with dual fluorescent reporters, created by targeted insertion at the Igs7 locus (TIGRE). Exposure to Cre recombinase removes both STOP cassettes, resulting in tTA2 expression, robust tdTomato fluorescence and EGFP/shOct4 fusion protein expression (robust EGFP fluorescence and potential knockdown of endogenous Pou5f1 expression by short hairpin RNA shOct4). The EGFP/shOct4 expression may be expected to be diminished/eliminated by doxycycline.

型号：GM-J#034102 -Cryorecovery

应用：Ai174 (also called Ai174(TIT2L-EGFP-shHDAC1-ICL-tdT-tTA2)) is a Cre-dependent, Tet-controllable histone deacetylation tool strain with dual fluorescent reporters, created by targeted insertion at the Igs7 locus (TIGRE). Exposure to Cre recombinase removes both STOP cassettes, resulting in tTA2 expression, robust tdTomato fluorescence and EGFP/shHDAC1 fusion protein expression (robust EGFP fluorescence and potential knockdown of endogenous histone deacetylase 1 expression by short hairpin RNA shHDAC1). The EGFP/shHDAC1 expression may be expected to be diminished/eliminated by doxycycline.

型号：GM-J#034101 -Cryorecovery

应用：Ai173 (also called Ai173(TIT2L-EGFP-HDAC1-ICL-tdT-tTA2)) is a Cre-dependent, Tet-controllable histone deacetylation tool strain with dual fluorescent reporters, created by targeted insertion at the Igs7 locus (TIGRE). Exposure to Cre recombinase removes both STOP cassettes, resulting in tTA2 expression, robust tdTomato fluorescence and EGFP/HDAC1 fusion protein expression. HDAC1 is a potent epigenetic regulator and its overexpression will promote histone deacetylation. EGFP/HDAC1 expression may be expected to be diminished/eliminated by doxycycline.

型号：GM-J#034100 -Cryorecovery

应用：Ai140 (also called Ai140(TIT2L-EGFP-ICL-tTA2)) is a Cre-dependent, Tet-controllable fluorescent reporter line, created by targeted insertion at the Igs7 locus (TIGRE). Exposure to Cre recombinase removes both STOP cassettes - resulting in tTA2 expression and robust EGFP fluorescence. EGFP expression may be expected to be diminished/eliminated by doxycycline.

型号：GM-J#034099 -Cryorecovery

应用：Ai139 (also called Ai139(TIT2L-EGFP-ICL-TPT)) is a Cre-dependent, Tet-controllable dual fluorescent reporter line, created by targeted insertion at the Igs7 locus (TIGRE). Exposure to Cre recombinase removes both STOP cassettes resulting in tTA2 expression, robust EGFP fluorescence and robust tdTomato fluorescence. EGFP expression may be diminished/eliminated by doxycycline.

型号：GM-J#034000 -Cryorecovery

应用：Tcerg1l-2A-CreER knock-in mice express tamoxifen-inducible CreERT2 fusion protein from the mouse Tcerg1l (transcription elongation regulator 1-like) promoter in TCERG1L-positive neurons of the neocortex, hypothalamus and tectum.

型号：GM-J#033999 -Cryorecovery

应用：Adcyap1-2A-CreER knock-in mice express tamoxifen-inducible CreERT2 fusion protein from the mouse Adcyap1 (adenylate cyclase activating polypeptide 1) promoter in ADCYAP1-positive neurons of the neocortex and pons.