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  • B6;129S6-Igs7tm195(tetO-GCaMP7s,CAG-tTA2)

    产品型号:GM-J#034112 -Available

    产品应用:Ai195 (also called Ai195(TIT2L-GCaMP7s-ICF-IRES-tTA2)) is a dual-recombinase responsive (Cre/FLP-dependent), Tet-controllable, fluorescent calcium-indicator tool allele created by targeted insertion at the Igs7 locus (TIGRE). Exposure to both Cre and FLP recombinase results in expression of tTA2 and jGCaMP7s (an ultrasensitive EGFP fluorescent calcium detector of neuronal activity with slower decay and response kinetics) in those cells where Cre and FLP expression overlaps/intersects. The jGCaMP7s expression may be expected to be diminished/eliminated by doxycycline. jGCaMP7s has high sensitivity and affinity, improved detection of individual spikes and may allow tracking larger populations of neurons using two-photon imaging.

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