产品型号：GM-J#034103 -Cryorecovery

产品应用：Ai175 (also called Ai175(TIT2L-EGFP-shOct4-ICL-tdT-tTA2)) is a Cre-dependent, Tet-controllable transcription tool strain with dual fluorescent reporters, created by targeted insertion at the Igs7 locus (TIGRE). Exposure to Cre recombinase removes both STOP cassettes, resulting in tTA2 expression, robust tdTomato fluorescence and EGFP/shOct4 fusion protein expression (robust EGFP fluorescence and potential knockdown of endogenous Pou5f1 expression by short hairpin RNA shOct4). The EGFP/shOct4 expression may be expected to be diminished/eliminated by doxycycline.