型号：GM-J#024112 -Cryorecovery

应用：Ai94(TITL-GCaMP6s)-D;Rosa26-ZtTA (or Ai94D;Rosa26-ZtTA) mice have a Cre/Tet-dependent, fluorescent calcium indicator GCaMP6s inserted into the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated), as well as a Cre-dependent tetracycline-controlled transactivator protein (tTA) inserted into the Gt(ROSA)26Sor locus on chromosome 6. After removal of the floxed-STOP cassette by Cre recombinase, resulting mice have doxycycline-inducible/reversible expression of GCaMP6 slow variant calcium indicator (GCaMP6s; an ultrasensitive detector of single neuronal action potentials with slower decay and response kinetics). Following subsequent calcium binding (such as neuronal activation), increased EGFP fluorescence is observed in those cells.

型号：GM-J#024109 -Available Now

应用：Ai32 mice express an improved channelrhodopsin-2/EYFP fusion protein following exposure to Cre recombinase. These mice can be used in optogenetic studies for rapid in vivo activation of excitable cells by illumination with blue light (450-490 nm).

型号：GM-J#024108 -Cryorecovery

应用：Ai93(TITL-GCaMP6f)-D;CaMK2a-tTA (or Ai93D;CaMK2a-tTA) mice have a Cre/Tet-dependent, fluorescent calcium indicator GCaMP6f inserted into the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated), as well as a transgene directing tetracycline-controlled transactivator protein (tTA) expression in forebrain neurons. After removal of the floxed-STOP cassette by Cre recombinase, resulting mice have doxycycline-inducible/reversible expression of GCaMP6 fast variant calcium indicator (GCaMP6f; a detector of single neuronal action potentials with fast response kinetics) in forebrain neurons. Following subsequent calcium binding (such as neuronal activation), bright EGFP fluorescence is observed.

型号：GM-J#024107 -Cryorecovery

应用：Ai93(TITL-GCaMP6f)-D;Rosa26-ZtTA (or Ai93D;R26-ZtTA) mice have a Cre/Tet-dependent, fluorescent calcium indicator GCaMP6f inserted into the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated), as well as a Cre-dependent tetracycline-controlled transactivator protein (tTA) inserted into the Gt(ROSA)26Sor locus on chromosome 6. After removal of the floxed-STOP cassettes by Cre recombinase, resulting mice have doxycycline-inducible/reversible expression of GCaMP6 fast variant calcium indicator (GCaMP6f; a detector of single neuronal action potentials with fast response kinetics). Following subsequent calcium binding (such as neuronal activation), bright EGFP fluorescence is observed.

型号：GM-J#024106 -Cryorecovery

应用：This Ai96 allele is available on a C57BL/6J genetic background as Stock No. 028866 and that version will replace this mixed background strain in the future.

型号：GM-J#024105 -Cryorecovery

应用：This Ai95D allele is available on a C57BL/6J genetic background as Stock No. 028865; which replaced this mixed background strain.

型号：GM-J#024104 -Cryorecovery

应用：Ai94(TITL-GCaMP6s)-D (also called Ai94D) mice have a Cre/Tet-dependent, fluorescent calcium indicator GCaMP6s inserted into the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). After removal of the floxed-STOP cassette by Cre recombinase, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of GCaMP6 slow variant calcium indicator (GCaMP6s; an ultrasensitive detector of single neuronal action potentials with slower decay and response kinetics). Following subsequent calcium binding (such as neuronal activation), increased EGFP fluorescence is observed.

型号：GM-J#024103 -Cryorecovery

应用：Ai93(TITL-GCaMP6f)-D (also called Ai93D) mice are a Cre/Tet-dependent, fluorescent calcium indicator line, created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). After removal of the floxed-STOP cassettes by Cre recombinase, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of GCaMP6 fast variant calcium indicator (GCaMP6f; a detector of single neuronal action potentials with fast response kinetics). Following subsequent calcium binding (such as neuronal activation), bright EGFP fluorescence is observed.

型号：GM-J#024101 -Cryorecovery

应用：The interleukin 1 type I receptor restore allele (IL-1R1r) is a functional null prior to Cre recombinase exposure. These mice allow Cre recombinase-inducible restoration of functional IL-1R1 expression, and visualization of IL-1R1-expressing cells with concordant labeling of IL-1R1 mRNA (by tdTomato fluorescence) and protein (by 3xHA epitope). IL-1R1r mice may be useful for studying cell-type-specific functions of IL-1R1 in neuroimmunity and neuroinflammation.

型号：GM-J#024100 -Cryorecovery

应用：Ai90(TITL-Chronos)-D (also called Ai90D) mice are a Cre/Tet-dependent, optogenetic line, created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). Following Cre-mediated removal of the STOP cassette, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of the channelrhodopsin/EGFP fusion protein (Chronos/EGFP). Subsequent illumination of Chronos-expressing (EGFP fluorescent) cells with blue-to-green light leads to reversible photostimulation of action potential firing/neural activity in these cells.