产品型号：GM-J#024108 -Cryorecovery

产品应用：Ai93(TITL-GCaMP6f)-D;CaMK2a-tTA (or Ai93D;CaMK2a-tTA) mice have a Cre/Tet-dependent, fluorescent calcium indicator GCaMP6f inserted into the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated), as well as a transgene directing tetracycline-controlled transactivator protein (tTA) expression in forebrain neurons. After removal of the floxed-STOP cassette by Cre recombinase, resulting mice have doxycycline-inducible/reversible expression of GCaMP6 fast variant calcium indicator (GCaMP6f; a detector of single neuronal action potentials with fast response kinetics) in forebrain neurons. Following subsequent calcium binding (such as neuronal activation), bright EGFP fluorescence is observed.