型号：GM-J#031570 -Cryorecovery

应用：Ai170(TIT2L-ASAP2s-Kv-ICL-tTA2)-D (or Ai170D) mice are a Cre/Tet-dependent, voltage-sensitive, fluorescent line for neuronal soma and proximal dendrites - created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). Exposure to Cre recombinase removes both STOP cassettes - resulting in expression of tTA2 and ASAP2s-Kv (highly-sensitive green fluorescent voltage indicator with Kv2.1-tag). ASAP2s-Kv expression may then be diminished by doxycycline. Ai170D mice may be useful for in vivo imaging of voltage dynamics in soma and proximal dendrites.

型号：GM-J#031569 -Cryorecovery

应用：Ai169(TIT2L-ASAP2s-ICL-tTA2)-D (or Ai169D) mice are a Cre/Tet-dependent, voltage-sensitive, fluorescent line - created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). Exposure to Cre recombinase removes both STOP cassettes - resulting in expression of tTA2 and ASAP2s (green fluorescent voltage indicator with high sensitivity). ASAP2s expression may then be diminished by doxycycline. Ai169D mice may be useful for in vivo imaging of voltage dynamics.

型号：GM-J#031565 -Cryorecovery

应用：Bap1lacZ mice express a β-galactosidase gene and are null for Bap1, in addition, exons 6-12 are flanked by loxP sites in this conditional knock-out-first strain. These mice may be useful in studies of clear cell renal carcinoma and von Hippel-Lindau syndrome.

型号：GM-J#031562 -Available

应用：Ai162(TIT2L-GC6s-ICL-tTA2)-D (also called Ai162D) mice are a Cre-dependent, Tet-controllable, fluorescent calcium-indicator tool strain - created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). Exposure to Cre recombinase removes both STOP cassettes, resulting in expression of tTA2 and GCaMP6s (an ultrasensitive detector of single neuronal action potentials with slower decay and response kinetics). GCaMP6s expression may then be diminished by doxycycline.

型号：GM-J#031561 -Cryorecovery

应用：Ai161(TIT2L-GFP-ICR-tTA2)-D (also called Ai161D) mice are a dual-recombinase responsive (Dre and Cre-dependent), Tet-controllable fluorescent reporter line, created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). Exposure to Dre recombinase removes the rox-flanked STOP cassette and results in tTA2 expression. Subsequent exposure to Cre recombinase removes the loxP-flanked STOP cassette - allowing the tTA2 to induce EGFP expression. The EGFP expression may then be diminished by doxycycline. Ai161D mice allow intersectional genetic fate mapping of different cell subpopulations defined by the overlap of the two gene promoters driving the expression of each recombinase.

型号：GM-J#031520 -Cryorecovery

应用：Tbx18-CreERT2 knock-in/knock-out has the endogenous T-box18 promoter/enhancer sequences directing expression of tamoxifen-inducible Cre recombinase. These mice allow specific and inducible genetic manipulations in vivo for studying pericytes and vascular smooth muscle cells in multiple tissues, as well as proepicardium/epicardium cell lineages and pacemaker cells of the sino-atrial node.

型号：GM-J#031519 -Cryorecovery

应用：Tbx18:nlacZ/nGFP knock-in/knock-out has the endogenous T-box18 promoter/enhancer sequences directing expression of nuclear-localized lacZ before exposure to Cre recombinase. Subsequent Cre recombination results in nuclear GFP fluorescence. These mice may be useful in studying pericytes and vascular smooth muscle cells in multiple tissues, as well as proepicardium/epicardium cell lineages, cardiac fibroblasts and pacemaker cells of the sino-atrial node.

型号：GM-J#031487 -Cryorecovery

应用：Agtr1a-2A-Cre knock-in mice are designed to have Cre recombinase expression directed to Agtr1a-expressing cells. Cre activity recapitulates that of the endogenous Agtr1a gene in the brain. These mice may be used to generate conditional mutations for studying neural circuits underlying thirst, vasopressin secretion, and fluid homeostasis.

型号：GM-J#031485 -Cryorecovery

应用：This knock-in/knock-out strain, backcrossed to C57BL/6J from the original mixed genetic background (see Stock No. 030357), expresses cre recombinase from the Pou4f2 promoter. In the original line, cre expression is observed in the retina, brain, intestines, heart, and gonads.

型号：GM-J#031437 -Cryorecovery

应用：Tcf4CreERT2 knock-in/knock-out mice express an inducible Cre/ERT2 fusion protein in connective tissue fibroblasts.