型号：GM-J#017923 -Cryorecovery

应用：The Miya10GT (M10GT) transgene has the CMV enhancer/chicken beta-actin core promoter upstream of a frt-flanked "MADM GT" cassette, all inserted into an intergenic region on chromosome 10 (~21 Mbp; between the Ahi1 and Myb loci). These mutant mice are designed for MADM-10 (mosaic analysis with double markers on chromosome 10), and provide a tool to generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows Cre recombinase-induced fluorescent labeling of daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.

型号：GM-J#017922 -Cryorecovery

应用：The R26TT allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM TT" cassette inserted into the Gt(ROSA)26Sor locus on chromosome 6. These R26TT mice exhibit widespread expression of tdTomato-3Myc (tdT3Myc) and represent the tdTomato-expressing control strain for "new MADM-6" (new mosaic analysis with double markers on chromosome 6) experiments.

型号：GM-J#017921 -Cryorecovery

应用：The R26TG allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM TG" cassette (ATG-intron-GFPC-terminus) inserted into the Gt(ROSA)26Sor locus on chromosome 6. The new TG cassette in R26TG mice is compatible with any "GX cassette" (GFPN-terminus-intron-XATG-less) where XATG-less is any gene without the start codon. Like the original MADM system, the "new MADM-6" system provides a tool to generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows Cre or FLP recombinase-induced fluorescent labeling of daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis, as well as for the MADM-Tet system.

型号：GM-J#017919 -Cryorecovery

应用：The preinduction parent Tg mouse line (amiR-TDP43u line 6 or CAG-loxp-EGFP/3xpA-loxp-RFP-amiR-TDP43) has a loxP-flanked EGFP/STOP cassette preventing transcription of a downstream red fluorescent protein (dsRed2) and an artificial miRNA (amiR-TDP43) that targets the mouse TAR DNA binding protein mRNA for knockdown. Once induced by Cre recombinase, amiR-TDP43 expression in CNS, spinal cord and muscle results in reduced TDP-43 expression and a rapid, progressive neurodegenerative phenotype that recapitulates many features of human amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD).

型号：GM-J#017915 -Cryorecovery

应用：The PRCre knockin allele harbors an internal ribosome entry site and Cre recombinase gene downstream of the progesterone receptor (Pgr or PR) transcriptonal stop codon. As such, Cre recombinase expression is directed to Pgr-expressing cells by the endogenous promoter/enhancer elements of the progesterone receptor locus. These PRCre knockin mice may be useful for studying the function of this ligand-inducible transcription factor in progesterone-responsive cells/tissues such as uterus, ovary, oviduct, pituitary gland, and mammary gland. They may also be useful for studying sexually dimorphic nuclei in the hypothalamus and steroid hormone-induced malignancies.

型号：GM-J#017913 -Cryorecovery

应用：The Esr1cre knockin allele has Cre recombinase expression directed to estrogen receptor 1 alpha (Esr1 or ERα)-expressing cells by the endogenous Esr1 promoter/enhancer elements; specifically in the ventrolateral subdivision of the ventromedial hypothalamus that, like wildtype mice, is greater in females than males. Expression is also observed in arcuate nucleus (ARH). These Esr1-Cre knockin mice may be useful for studying the function of this ligand-inducible transcription factor in social interactions, neuronal differentiation, sympathetic nervous system development, hypothalamus, female reproductive organs (endometrium and ovarian stroma cells), and male efferent duct epithelial cells. They may also be useful for studying ERα-positive breast cancer and steroid hormone-induced malignancies. Esr1-Cre knockin mice are available on a C57BL/6N background (Stock No. 017911) and a C57BL/6J background (Stock No. 017913).

型号：GM-J#017912 -Cryorecovery

应用：The R26GT allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM GT" cassette (GFPN-terminus-intron-tdT3MycATG-less) inserted into the Gt(ROSA)26Sor locus on chromosome 6. Like the original MADM system, this "new MADM-6" system provides a tool to generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows Cre or FLP recombinase-induced fluorescent labeling of daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.

型号：GM-J#017911 -Cryorecovery

应用：The Esr1cre knockin allele has Cre recombinase expression directed to estrogen receptor 1 alpha (Esr1 or ERα)-expressing cells by the endogenous Esr1 promoter/enhancer elements; specifically in the ventrolateral subdivision of the ventromedial hypothalamus that, like wildtype mice, is greater in females than males. Expression is also observed in arcuate nucleus (ARH). These Esr1-Cre knockin mice may be useful for studying the function of this ligand-inducible transcription factor in social interactions, neuronal differentiation, sympathetic nervous system development, hypothalamus, female reproductive organs (endometrium and ovarian stroma cells), and male efferent duct epithelial cells. They may also be useful for studying ERα-positive breast cancer and steroid hormone-induced malignancies. Esr1-Cre knockin mice are available on a C57BL/6N background (Stock No. 017911) and a C57BL/6J background (Stock No. 017913).

型号：GM-J#017909 -Cryorecovery

应用：The R26G-tTA2 allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM G-tTA2" (GFPN-terminus-intron-tTA2ATG-less) inserted into the Gt(ROSA)26Sor locus on chromosome 6. These mutant mice are designed to combine the "new MADM-6" system with a Tet-Off binary expression system to create MADM-Tet (mosaic analysis with double markers combined with Tet-Off). This is a tool to generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows Cre or FLP recombinase-induced fluorescent labeling/tTA2-expression in daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice are also a Tet-Off tool enabling a TRE transgene to be conditionally expressed in a subset of MADM-labeled cells.

型号：GM-J#017862 -Cryorecovery

应用：These pTH-ferritin transgenic mice exhibit increased ferritin-bound iron levels in the brain, decreased ferrous iron levels in the substantia nigra, and reduced sensitivity to MPTP-induced Parkinson's disease-like symptoms. They have applications in studies related to iron deficiency and Parkinson's disease.