型号：GM-J#021188 -Available

应用：These Ai40D mice conditionally express an improved Archaerhodopsin-3/EGFP fusion protein (ArchT-EGFP) from the endogenous Gt(ROSA)26Sor locus. Expression is enhanced by the presence of a CAG promoter. Following Cre-mediated removal of the floxed-STOP cassette, ArchT-EGFP expression is observed in the cre-expressing cells. Subsequent illumination of these cells with yellow-green light (~575 nm) leads to reversible photoinhibition of action potential firing/neural activity in these cells. These Ai40D mice are useful for optogenetic studies to express an inhibitory opsin that effectively silences the activity of cortical neurons (and perhaps other excitable cell types such as muscle cells and immune cells). ArchT has greater than 3-fold improvement in light sensitivity compared to Arch, equating to greater than 200% increase in brain tissue volume addressed by a typical single optical fiber.

型号：GM-J#021162 -Available

应用：Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from a cryorecovery.

型号：GM-J#021160 -Available

应用：Cx3cr1CreER knock-in/knock-out mice express a Cre-ERT2 fusion protein and EYFP in microglia in the brain. These mice may have applications in studies requiring conditional gene manipulation in central nervous system microglia, as well as other Cx3cr1-expressing myeloid cell populations.

型号：GM-J#021077 -Cryorecovery

应用：The entire open reading frame of the Mrgprb4 (MAS-related GPR, member B4) gene was replaced by an mtdTomato-2A-NLSCre-Frt-PGK-Neomycin-FRT cassette in these knockout/knockin allele mutant mice. When virally transduced, they are useful in studies of Mrgprb4-expressing sensory neurons. Constitutive expression of mtdTomato has been demonstrated in dorsal root ganglion (DRG) cells.

型号：GM-J#021055 -Cryorecovery

应用：These mice possess loxP sites flanking exon 5 of the brain derived neurotrophic factor (Bdnf) gene, as well as a Cre recombinase-mediated beta-galactosidase reporter and have applications in studies of neuronal survival and differentiation, synaptic function and behavior.

型号：GM-J#021039 -Available

应用：These Sun1-tagged mice are used in the INTACT (isolation of nuclei tagged in specific cell types) method for immunopurification of nuclei. Cre-dependent removal of a floxed STOP cassette allows expression of the SUN1 fusion protein at the inner nuclear membrane in targeted cell types. These nuclei can be immunopurified with antibodies against GFP or MYC for transcriptional and epigenetic studies.

型号：GM-J#021025 -Cryorecovery

应用：These double mutant R26-M2rtTA::TetOP-Cre mice utilize the rtTA/tetO (tet-on/TRE) system to allow highly-efficient, doxycycline-inducible Cre recombinase expression in hematopoietic stem cells. These mice harbor the R26M2rtTA targeted mutation (an optimized form of reverse tetracycline controlled transactivator [rtTA-M2] downstream of the Gt(ROSA)26Sor promoter) and the Col1a1TetOP-Cre targeted mutation (a tetracycline responsive element-driven Cre recombinase targeted to the collagen type I alpha 1 locus).

型号：GM-J#021024 -Cryorecovery

应用：Csf1r-iCre transgenic mice have codon optimized Cre recombinase (iCre) expression under direction of the murine colony stimulating factor 1 receptor promoter (Csf1r). iCre recombinase expression is evident in immune cells, including macrophages, dendritic cells and bone marrow derived granulocytes.

型号：GM-J#021012 -Cryorecovery

应用：These transgenic mice carry the Brainbow 1.0 cassette, incorporating dTomato, mCerulean, and EYFP, driven by the broadly-expressed CAG (CMV-chicken beta actin) promoter. These fluorescent markers are useful in tracing cell lineage.

型号：GM-J#021011 -Cryorecovery

应用：These transgenic mice carry the Brainbow 1.0 cassette, incorporating dTomato, mCerulean, and EYFP, driven by the broadly-expressed CAG (CMV-chicken beta actin) promoter. These fluorescent markers are useful in tracing cell lineage.