产品型号：GM-J#021188 -Available

产品应用：These Ai40D mice conditionally express an improved Archaerhodopsin-3/EGFP fusion protein (ArchT-EGFP) from the endogenous Gt(ROSA)26Sor locus. Expression is enhanced by the presence of a CAG promoter. Following Cre-mediated removal of the floxed-STOP cassette, ArchT-EGFP expression is observed in the cre-expressing cells. Subsequent illumination of these cells with yellow-green light (~575 nm) leads to reversible photoinhibition of action potential firing/neural activity in these cells. These Ai40D mice are useful for optogenetic studies to express an inhibitory opsin that effectively silences the activity of cortical neurons (and perhaps other excitable cell types such as muscle cells and immune cells). ArchT has greater than 3-fold improvement in light sensitivity compared to Arch, equating to greater than 200% increase in brain tissue volume addressed by a typical single optical fiber.