产品型号：GM-J#006953 -Cryorecovery

产品应用：N1::cre mutant mice express a Notch1-Cre fusion protein formed by the insertion of Cre recombinase into the Notch1 locus, replacing the intracellular domain (NICD1).Interaction of N1::cre fusion receptors in vivo with Notch-DSL ligands results in proteolytic cleavage of the transmembrane domain tether and subsequent release of Cre recombinase protein from the plasma membrane. When bred to mice with a loxP-flanked reporter sequence, cell descendants have differential Cre-mediated reporter protein labeling; Notch1 signaling in actively cycling stem/progenitor cells will mark all their descendants producing a "clone", whereas Notch1 activation in transit amplifying or differentiating cells will result in small clones (2-4 cells) or in salt-and-pepper patterns of individually labeled cells. These N1::cre mice make it possible to follow the deccendents of cells that have undergone Notch1 activation, throughout development, self-renewal and malignancy.