产品型号：GM-J#029899 -Available

产品应用：The NuTRAP allele (Nuclear tagging and Translating Ribosome Affinity Purification) has a loxP-flanked STOP preventing transcription of three individual components: BirA, BLRP-tagged mCherry/mRANGAP1 and EGFP/L10a. When expressed, the BLRP-tagged mCherry/mRANGAP1 protein is biotinylated by BirA, allowing nuclear membrane-labeling with mCherry and biotin (which enables nuclear isolation by fluorescence- and affinity-based purification). Furthermore, EGFP/L10a fluorescently tags the translating mRNA polysome complex (which enables isolation of RNAs that are actively engaged by ribosomes). Overall, NuTRAP mice are a Cre-inducible tool strain that allows labeling and simultaneous isolation of cell type-specific nuclei and mRNA, and are well-suited for studying epigenomics and transcriptomics from specific cell types within a heterogeneous tissue. Of note, the donating investigator also has available their similar mouse line H2B-TRAP (Stock No. 029789).