产品型号：GM-J#026942 -Available

产品应用：RC::FL-hM3Dq is a dual recombinase-responsive Gq-coupled DREADD allele with a frt-flanked STOP and a cre-dependent FLEx switch containing an eGFP sequence and an inverted hM3Dq/mCherry/2ACT88 fusion protein. Flp-recombinase results in widespread, robust eGFP fluorescence, and further exposure to Cre recombinase may replace eGFP expression with somatodendritic hM3Dq/mCherry expression. The DREADD hM3Dq induces the canonical Gq pathway (depolarization/activation) specifically following administration of its pharmacologically inert ligand (CNO). The RC::FL-hM3Dq allele and its derivatives permit chemogenetic/pharmacogenetic activation of an intersectional subpopulation defined by overlap of Flp and Cre recombinase expression.