产品型号：GM-J#026260 -Cryorecovery

产品应用：Ai85(TITL-iGluSnFr)-D (also called Ai85D) mice are a Cre/Tet-dependent, fluorescent glutamate indicator line, created by targeted insertion of floxed-STOP-iGluSnFr at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). After removal of the floxed-STOP cassette by Cre recombinase, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of iGluSnFr, an intensity-based glutamate-sensing fluorescent reporter that is fast and sensitive. Increased GFP fluorescence is observed following subsequent glutamate binding (such as excitatory synaptic activity).