产品型号：GM-J#025109 -Cryorecovery

产品应用：The Ai80(RCFL-CatCh)-D (or Ai80D) conditional allele has both an frt-flanked STOP cassette and a loxP-flanked STOP cassette preventing transcription of the calcium translocating channelrhodopsin (CatCh; an L132C mutant channelrhodopsin with enhanced Ca2+ permeability, accelerated response kinetics and ~70-fold greater light sensitivity) and EYFP. After removal of the flanked STOP cassettes by FLP- and Cre-recombination, CatCh/EYFP expression is expected in cells/tissues expressing both FLP recombinase and Cre recombinase. Subsequent illumination of CatCh-expressing cells with blue-to-green light leads to reversible photostimulation of action potential firing/neural activity in these cells.