产品型号：GM-J#023528 -Cryorecovery

产品应用：Ai78(TITL-VSFPB)-D (also called Ai78D) mice are a Cre/Tet-dependent, voltage-sensitive, fluorescent line, created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). After removal of the floxed-STOP cassette by Cre recombinase, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of the voltage-sensitive fluorescent protein Butterfly 1.2 (VSFP-Butterfly1.2 or VSFPB1.2). VSFP-Butterfly1.2 exhibits voltage-dependent FRET upon membrane depolarization, and enables optical recordings at high spatial resolution with intrinsic assignment of recorded signals to VSFP-targeted cell populations (such as layer 2/3 pyramidal cells).